Overview
- Affinity purification Poly-His-tagged recombinant proteins.
- High binding capacity
- Super porous matrix suitable for large proteins and protein complexes.
- High resistant to reducing agents such as DTT, BME and TCEP.
- High tolerance to EDTA and other chelating agents.
- Regenerable.
Binding Capacity | 30-40 mg/mL of middle size proteins |
Matrix | Highly crosslinked 4% agarose beads |
Ligand | NTA |
Bead Diameter | 40-90 um |
Compatible Buffers | Common aqueous buffer with pH above 7.0 |
Tolerance of Reducing Agents | Up to 10 mM BME, 5 mM DTT or 5 mM TCEP |
Tolerance of EDTA | Up to 10 mM EDTA |
Tolerance of Urea/Guanidine | 8 M Urea or 6 M Guanidine HCL |
Tolerance of NaOH | 0.1 - 0.5 M NaOH CIP |
Storage | 4-10 ˚C, with 20% isopropanol |
