Antibody/IgG Purification
Antibody molecules can be enriched and concentrated from natural animal serum or supernatant of a recombinant expression system. Affinity chromatography with Protein A/G resins or ion exchange chromatography with cationic/anionic resins are generally used. We have developed several affinity agarose resins for efficient isolation and purification of IgG antibodies.
His-tagged Protein Purification
Poly-Histidine tag is the most used protein tag for recombinant proteins. Our IMAC resins have NTA and IDA ligands for isolation of His-tagged proteins at a single step.
Ion Exchanged Based Purification
The surface of a protein molecule may be positively or negatively charged under certain condition. And the surface may also have patches of positive and negative charge. Ion exchange resins can be used to bind charged protein molecules for separation purposes.
Heparin Binding Protein Purification
Heparin is a positively charged polysaccharide. A number of proteins are specifically attracted by Heparin. For examples, DNA/RNA binding proteins, cytokines, plasma factors and etc. can be efficiently purified with Heparin resins.
Streptavidin-Biotin Interaction
Recombinant Streptavidin is directionally immobilized on our super porous agarose resin. Our Streptavidin resin has higher binding capacity for biotinylated proteins than most of the similar commercially available products.
Hydrophobic Interaction Chromatography HIC
HIC resins are used to purify proteins with a hydrophobic surface or patches.
Endotoxin Removal
Endotoxins are components of cell wall of gram-negative bacteria and can have contaminate recombinant proteins, DNA/RNA prep and any biological products. We have developed several endotoxin adsorbent to bind endotoxins in solutions.
Antibody/Protein/Peptide Immobilization
Immobilization of a particular protein, enzyme or antibody to a solid support such as agarose beads, magnetic beads or membrane is a common practice for assays. We have several ready-to-go beads which can be easily used to chemically attach a protein/antibody/peptide of interest.